Use this url to cite publication: https://hdl.handle.net/20.500.12259/60770
Options
Cold plasma treatment as seed dormancy breaking agent
Type of publication
Konferencijų tezės nerecenzuojamame leidinyje / Conference theses in non-peer-reviewed publication (T2)
Author(s)
LT | ||
LT | ||
LT | ||
Pančenka, Olegas | LT | |
LT | ||
LT | ||
LT | ||
Koga, Kazunori | Kyushu university, Fukuoka, Japan | JP |
Shiratani, Masaharu | Kyushu university, Fukuoka, Japan | JP |
Title
Cold plasma treatment as seed dormancy breaking agent
Is part of
Smart Bio : ICSB 2nd international conference, 3-5 May 2018, Kaunas : abstracts book. Kaunas : Vytautas Magnus University, 2018
Date Issued
Date Issued |
---|
2018 |
Publisher
Kaunas : Vytautas Magnus University, 2018
Publisher (trusted)
CEEOL |
Extent
p. 128-128
Field of Science
Abstract
The aim of this study was to evaluate the dependence of atmospheric cold plasma (CP) irradiation effects on the duration of treatment and time interval passed between treatment and sowing. We estimated changes induced in radish (Raphanus sativus) germination and content of the main phytohormones controlling germination – abscisic acid (ABA) and gibberelins (GA7 and GA3) in dry seeds. Radish seeds were treated for different durations (1, 3, 5, 7 and 10 min) with a scalable dielectric barrier discharge (DBD) device. The discharge voltage, current and power density were 7.0 kV, 0.2 A and 3.1 W/cm2, respectively. Germination tests in vitro were performed 0, 1, 4, 8 days (CP 5 min) and 14 days (CP 1, 2, 3, 5, 7, 10 min) after treatment, and at the same time methanol extracts of dry seed homogenates were prepared for phytohormone HPLC analysis. Indices of germination dynamics – the final germination percentage Vi (%) and the median germination time Me (h), were determined using Richards plots. Morphometric seedling analysis was performed 4 days after sowing. CP treatment strongly stimulated germination: Vi increased from 18 to 30%; Me decreased from 15 to 29% in all CP treated seed groups in comparison to control. Increase of irradiation time above 5 min did not lead to stronger positive effects on germination as it was achieved by 5 min irradiation. Both effects on germination and morphometric parameters of seedlings were stronger when time interval between seed treatment and sowing was increased from 0 or 1 day to 4 days. In all groups of CP-treated seeds ABA content was strongly reduced while GA7 content was increased. Strong negative correlation (R2=0,934) was obtained between Vi and ABA content, and between Vi and ABA/(GA7+GA3) (R2=0,936). Positive polynomial correlation between Me and GA7+GA3 was characterized by R2=0,843. [...]
Type of document
type::text::conference output::conference proceedings::conference paper
Language
Anglų / English (en)
Coverage Spatial
Lietuva / Lithuania (LT)